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href="home.php?mod=space&uid=54876">作者：update</a> 阅读<em id="_viewnum">(49)</em> 评论: <a href="http://www.oecr.com/portal.php?mod=comment&id=10239&idtype=aid" title="查看全部评论"><em id="_commentnum">5</em></a></p> <div class="zxfb" ><a href="http://www.oecr.com/portal.php?mod=rss&catid=57" class="y xi2 rss" target="_blank" title="RSS">订阅</a></div> </div> <!--[diy=diysummarytop]--><div id="diysummarytop" class="area"></div><!--[/diy]--> <div class="s"> <div class="ss"><div><span>导读： </span>一种新型功能化荧光传感器可实时高灵敏度检测肠道病毒RNA 。这标志着更安全、更有效的病毒诊断迈出了重要一步。芬兰于韦斯屈莱大学纳米科学中心 (NSC) 的研究人员取得了一项重大突破，开发出一种创新的无标记比例荧 ...</div> </div> <!--[diy=diysummarybottom]--><div id="diysummarybottom" class="area"></div><!--[/diy]--> <div class="d"> <!--[diy=diycontenttop]--><div id="diycontenttop" class="area"></div><!--[/diy]--> <table cellpadding="0" cellspacing="0" class="vwtb"><tr><td id="article_content"><div> 一种新型功能化荧光<a href="http://sensor.oecr.com" target="_blank" class="relatedlink">传感</a>器可实时高灵敏度检测肠道病毒RNA。这标志着更安全、更有效的病毒诊断迈出了重要一步。芬兰于韦斯屈莱大学<a href="http://material.oecr.com" target="_blank" class="relatedlink">纳米</a>科学中心(NSC)的研究人员取得了一项重大突破，开发出一种创新的无标记比例荧光传感器，能够高灵敏度和高选择性地检测肠道病毒RNA。</div><div><br></div><div><p style="text-align: center;"><a href="data/attachment/portal/202504/11/090503myyd68x0d6960yay.jpg" target="_blank"><img src="data/attachment/portal/202504/11/090503myyd68x0d6960yay.jpg"></a></p></div><div style="text-align: center;"><br></div><div style="text-align: center;"> 基于功能化碳点(CD)和溴化乙锭(EB)的比例型荧光传感器（Func传感器）的工作原理图。图片来源：JussiToppari教授绘制</div><div><br></div><div> 这一进展代表了病毒诊断领域的重大进步，并凸显了跨学科合作在应对全球健康挑战中的价值。</div><div><br></div><div> 病毒持续对公众健康构成严重威胁，正如近期的疫情大流行所见。早期发现和准确识别对于控制感染的传播至关重要。传统的诊断方法虽然有效，但它们往往无法提供有关病毒基因组释放的详细时空信息，从而限制了其在动态或早期检测场景中的应用。</div><div><br></div><div> “这项跨学科的研究融合了生物学、化学和<a href="http://theory.oecr.com" target="_blank" class="relatedlink">物理</a>学的专业知识，标志着病毒检测技术的重大进步。我们开发了一种增强型比率荧光传感器，该传感器使用探针（单链互补寡核苷酸片段）和溴化乙锭（EB）功能化的碳点（CD），用于检测肠道病毒RNA。”于韦斯屈莱大学物理学教授JussiToppari说道。</div><div><br></div><div> 荧光纳米粒子已成为生物分析物传感的有力工具，而碳点凭借其简单的合成、优异的光稳定性、可调的光致<a href="http://light.oecr.com" target="_blank" class="relatedlink">发光</a>、优异的水溶性、生物相容性以及用于配体结合的多种表面功能，在生物传感领域处于领先地位。这些独特的特性使碳点有望成为生物传感领域的颠覆者。</div><div><br></div><div> “这种所谓的功能化传感器（FuncSensor），其中CD被功能化，即与探针共价结合，明显优于更传统的非功能化传感器（Non-FuncSensor），后者是CD、探针和EB的简单混合物，”于韦斯屈莱大学博士研究员AmarRaj解释道。</div><div><br></div><div> 在这两种传感器中，目标DNA与探针杂交，增强了EB荧光，而CD荧光由于<a href="http://electronic.oecr.com" target="_blank" class="relatedlink">电子</a>转移而略有变化，从而实现比率检测，并且具有超灵敏性。</div><div><br></div><div> 博士后研究员AbhishekPathak表示：“非功能性传感器对目标DNA的灵敏度较低，对真正的肠道病毒RNA样本无效；而功能性传感器对DNA和真正的病毒RNA的灵敏度更高，选择性明显提高。”他之前曾在于韦斯屈莱大学担任博士后研究员。</div><div><br></div><div> Func传感器的卓越性能归功于共价功能化带来的增强电荷转移。</div><div><br></div><div> “我们的原理验证研究强调了探针共价固定对于改善CD和EB之间电子转移的重要性，从而提高了性能，并证明了Func传感器在快速、实时和精确原位检测病毒RNA的实际应用中的适用性，”于韦斯屈莱大学细胞和分子生物学教授VarpuMarjomäki说道。</div><div><br></div><div> 尤其是研究表明Func传感器可以在体外实时检测衣壳中肠道病毒RNA的释放。Marjomäki说：“这意味着Func传感器可以用作一种新型病毒RNA传感平台，为检测感染期间实时病毒RNA的出现提供了急需的可能性。”</div><div><br></div><div> 这项突破性的研究引入了一种检测病毒RNA的新方法，并为荧光团之间的电荷转移机制提供了新的见解。基于这些发现，该团队目前正致力于用更安全、细胞毒性更低、生物相容性更高的替代品取代具有潜在危险性的染料溴化乙锭，从而提高该系统的安全性和可靠性。</div><div><br></div><div> Pathak高兴地表示：“这一改进将进一步提高体内病毒RNA检测的安全性和有效性。”</div><div><br></div><div><p microsoft="" yahei",="" simsun;="" background-color:="" rgb(255,="" 255,="" 255);="" text-align:="" center;"="" 255);"="" center;="" style="word-wrap: normal; margin: 0px; padding: 0px; color: rgb(143, 143, 143); font-family: Tahoma, "Microsoft Yahei", Simsun; background-color: rgb(255, 255, 255); text-align: center;"><a href="http://www.oecr.com/data/attachment/portal/202404/12/093958rszee6k8hhykve3w.jpg" target="_blank" style="word-wrap: normal; color: rgb(51, 51, 51); text-decoration: none; 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